Detection of viral rna not only aids in the diagnosis of illness but also provides. Is there a maccompatible control software for the stepone. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative. Intended for laboratory staff and principal investigators who perform experiments using the stepone or steponeplus system. The principle of real time pcr, reverse transcription, quantitative rt pcr duration. Applied biosystems stepone and steponeplus realtime pcr systems getting started guide for relative standard curve and comparative c t 4376785 c t experiments applied biosystems stepone and steponeplus realtime pcr systems getting started guide for standard curve experiments 4376784. The steponeplus realtime pcr system is a 96well realtime pcr instrument perfect for both firsttime and experienced users. Is there a maccompatible control software for the stepone rt pcr. Stepone and steponeplus realtime pcr system thermo.
Field application specialist doug rains offers advices for fixing common software setup mistakes when performing real time pcr. Depending on the experimental design, the system can even help you select and order realtime pcr reagents online by means of convenient links in the software optional. Amplify sample with the steponeplus real time system. I want to design real time pcr primers for my gene of interest which is 1kb in size and with one intron. The steponeplus realtime pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Applied biosystems stepone qpcr comes with its own software for template input and data output but this is only for pc. The system provides twofold discrimination with 99.
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